Application

Western Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot detected phospho PKCepsilon (Ser729) in 10 µg of NIH/3T3 cell lysate.

Anti-phospho-PKCepsilon Antibody (Ser729) is a highly specific rabbit polyclonal antibody, that targets PKC epsilon & has been tested in western blotting.

General description

Protein Kinase C epsilon (PKC epsilon) is an ~87 kDa (migrates predominantly as 95 kDa in quiescient cells) member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC epsilon is an actin cytoskeleton regulating protein whose overexpression confers an oncogenic phenotype by affecting the ras signaling cascade. PKC epsilon is phosphorylated by PDK1 on threonine 566 and is then autophosphorylated on the turn motif threonine 710 and the hydrophobic motif serine 729. Catalytically inactive PKC epsilon is not phosphorylated on serine 729. Phosphorylation of serine 729 is mediated by mTOR-dependent pathways, plays an important role in regulating PKC epsilon subcellular localization, and is responsible for the appearance of the 92 kDa PKC epsilon species.

Immunogen

KLH-conjugated linear peptide corresponding to human PKC phosphorylated at Ser729.

Epitope: Phosphorylated Ser729

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blotting/Peptide Inhibition Analysis in C6 cell lysate.

Western Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution of this lot detected phospho PKCepsilon (Ser729) in 10 µg of C6 cell lysate.

Specificity

This antibody demonstrates cross-reactivity against the PKCbeta (Ser661) isoform, PKCdelta (Ser655) isoform, and possibly PKCalpha (Ser729), all of which contain the GF(pS)Y motif.

Target description

~95 kDa and ~80 kDa observed. This protein has been observed at ~95 kDa and ~80 kDa (England, K. and Rumsby, M. G., et al. (2000). Biochem J. 352(Pt 1):19-26.).